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  • 1153
  • 12/07/2017

Ovarian tissue cryopreservation

Epublication WebSurg.com, Jul 2017;17(07). URL:
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The French law on bioethics dated August 6 2004 now states the possibility for any person to benefit from a gamete or a germinal tissue collection as well as its preservation. Collection and preservation are planned when the patient’s management may induce a fertility alteration or when the patient’s fertility may be prematurely altered. Young girls who have not reached the age of puberty and who are about to undergo high-dose chemotherapy and/or major radiation therapy for cancer, can benefit from a cryopreservation of their ovarian tissue. It will allow to preserve fertility when these patients have a wish for pregnancy. Once collected, preserved ovarian cortical strips are systematically sent for frozen section to the pathology department in order to be processed for cryopreservation. Later on, when these young women have been healed and wish to become pregnant, the thawing of preserved ovarian cortical strips and the orthotopic autograft can take place. We suggest to collect the anterior cortex from both ovaries, which allows to preserve the two remaining ovaries. Another technique, which is performed by other teams, involves a unilateral oophorectomy for ovarian tissue preservation. A bilateral hemicortical sampling on both ovaries is well accepted by patients and their parents. Indeed, the video does not present an oophorectomy, which is considered as a definitive procedure, but a bilateral sampling which leaves two ovaries in place. The ovaries are reduced in size but are anatomically and functionally healthy.